Three-dimensional Fluorescence Microscopy by Optical Scanning Holography

T-C. Poon, Virginia Polytechnic Institute and State Univ., Blacksburg, Va.

In optical sectioning microscopy (OSM), 3-D information is collected by recording a series of 2-D images at various focal planes throughout the 3-D specimen.1 However, during the recording of 2-D images, it is critical that exact longitudinal spacing between adjacent 2-D images and the precise registration of these image slides are accurately controlled. Scanning confocal microscopy (SCM) is a radically new microscope design.

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