Neuronal Activity in Mouse Primary Visual Cortex

In OPN's December “Optics in 2024” review of interesting research, scientists report the development of a novel high-speed two-photon adaptive line-excitation microscope that significantly enhances imaging speed while minimizing photodamage in neuronal imaging. The video shows in vivo imaging of the neuronal activity in mouse primary visual cortex. Left, high-resolution neuronal map; middle, high-speed neuronal activity recording captured by the two-photon microscope with the adaptive line-excitation scheme; right, extracted neuronal activity traces of representative neurons.