Feature

Objective for Fluorescence Microscopy

J. Brian Caldwell

One of the main problems in microscopy is that many objects of interest are invisible under ordinary lighting conditions. Most materials that are opaque and therefore easily seen in bulk form, become nearly transparent phase objects when reduced to sub-micron thickness. One technique for enhancing the contrast of microscopic subjects is to illuminate them with UV light and then observe the emitted fluorescent radiation.

Publish Date: 01 August 1998