Scatterings
Imaging Living Organisms with Sheets of Light
Caltech researchers developed a type of microscopy that simultaneously achieves high resolution, penetration depth and imaging speed, filling biologists’ need for fast 3-D imaging methods of organisms that don’t perturb the sample.
Three-dimensional live imaging of zebrafish showing both the embryo’s tissue (in white) and a fluorescent label driven by a gene-specific promoter (in orange).
Caltech researchers developed a type of microscopy that simultaneously achieves high resolution, penetration depth and imaging speed, filling biologists’ need for fast 3-D imaging methods of organisms that don’t perturb the sample. By combining two-photon excitation with light-sheet microscopy, Scott Fraser’s group demonstrated that the method provides deeper penetration than single-photon light-sheet microscopy. It also works much faster than point-scanning two-photon microscopy and doesn’t compromise normal biology (Nature Methods 8, 757; doi:10.1038/NMETH.1652).
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